The singer, 38, is known to have had problems with Aguilera many years ago, but has since insisted that they buried the hatchet.
And while Aguilera was performing a tribute to Whitney Houston, the camera cut to Pink – who then went viral for appearing to cringe at her.
However, she has spoken out on Twitter to deny that that was the case.
She wrote: “Christina f**king killed it tonight for one of our favorite singers ever.
“This about Whitney, and I am in awe of Christina’s talent. Show the clip where I’m in tears, you negative Nancy’s.”
Aguilera did not respond online to Pink, but did Like her tweet.
She also shared a video, in which she said: “To the AMAs, to Whitney’s beautiful family – thank you so much for this incredible opportunity, thank you so much for making a little girl’s dream come true.
“I’ve had the time of my life and I hope I did you proud, Whitney. Love you forever.”
Pink herself took to the stage twice last night: once on her own, and once as part of a duet with Kelly Clarkson, in which the pair sang REM’s Everybody Hurts in tribute to the emergency services.
Other notable performances came from a brave Selena Gomez, just months after a kidney transplant – but viewers cried foul by accusing her of miming.
Demi Lovato gave a well-received rendition of Sorry Not Sorry, Diana Ross delivered an epic medley, and Nick Jonas gave a rendition of Find You.
Big winners included Bruno Mars who scooped silverware for Artist of the Year, Favourite Pop/Rock Album, Favourite Pop/Rock Male Artist, Favourite Soul/R&B Male Artist, Video of the Year, Favourite Song – Soul/R&B.
Niall Horan took New Artist of the Year, Linkin Park took Favourite Artist – Alternative Rock, Coldplay scooped Tour of the Year and Collaboration of the Year was taken by Luis Fonsi and Daddy Yankee with Justin Bieber’s Despacito.
Despacito also won Favourite Song – Pop/Rock, while Drake was crowned Favourite Artist – Rap/Hip-Hop.
Lady Gaga won Favourite Female Artist – Pop/Rock, and Moana won Top Soundtrack.